A disconnect transfer conduit comprising flexible tubing a deformable sleeve surrounding a portion of the flexible tubing wherein the sleeve is attached to the flexible tubing and is formed of a material having plasticity such that pressure applied to the sleeve causes the sleeve to deform about and seal the flexible tubing and upon continued application of pressure to the sleeve, the sleeve and flexible tubing are cut and the sleeve retains a deformed shape substantially sealing the tubing. Also provided is a method of sealing flexible tubing comprising applying pressure to a sleeve attached to flexible tubing to deform the sleeve and seal the tubing wherein, applying further pressure to the sleeve to cut the sleeve and tubing, thereby leaving the tubing substantially sealed where cut.
