PROCÉDÉ DE CULTURE DE CELLULES SOUCHES SÉCRÉTANT DES COMPOSANTS ANTI-INFLAMMATOIRES, ET COMPOSITION ANTI-INFLAMMATOIRE CONTENANT LEDIT MILIEU DE CULTURE DE CELLULES SOUCHES
KIM, Joon Hong,김준홍,YUN, Sung Ji,윤성지,QUAN, Xiaoyuan,쵄쇼웬,CHOI, Jung Hye,최정혜
申请号：
KRKR2018/005317
公开号：
WO2019/216450A1
申请日:
2018.05.09
申请国别(地区):
KR
年份:
2019
代理人:
摘要：
An embodiment of the present invention provides: a method for culturing umbilical cord blood-derived pluripotent stem cells that secrete anti-inflammatory components for alleviating and treating inflammatory diseases； and an anti-inflammatory composition containing the stem cell culture medium. Specifically, the anti-inflammatory components can inhibit the amount of inflammatory substances such as nitric oxide (NO) and prostaglandin E2 (PGE2) produced, and more specifically, can inhibit the production of inflammatory substances by inhibiting the expression of iNOS and COX2 genes and proteins associated with inflammatory substances.L'invention concerne un mode de réalisation qui fournit : un procédé de culture de cellules souches pluripotentes dérivées du sang de cordon ombilical qui sécrètent des composants anti-inflammatoires pour soulager et traiter des maladies inflammatoires； et une composition anti-inflammatoire contenant le milieu de culture de cellules souches. En particulier, les composants anti-inflammatoires peuvent inhiber la quantité de substances inflammatoires telles que l'oxyde nitrique (NO) et la prostaglandine E2 (PGE2) produits, et plus spécifiquement, peut inhiber la production de substances inflammatoires par inhibition de l'expression de gènes et de protéines iNOS et COX2 associés à des substances inflammatoires.본 발명의 일실시예는 염증질환 개선 및 치료를 위한 항염증 성분을 분비하는 제대혈 유래 다분화능 줄기세포 배양방법 및 그 줄비세포 배양액을 포함하는 항염증조성물을 제공한다. 구체적으로 항염증 성분은 염증 유발 물질 NO(Nitric Oxide) 및 PGE2 (Prostaglandin E2)의 생성량을 억제할 수 있으며, 보다 더 구체적으로는 염증 유발 물질와 관려된 iNOS 및 COX2의 유전자 및 단백질 발현을 억제시킴으로써 염증 유발물질의 생성을 억제시킬 수 있다.
