Stem cells from human salivary glands, a process for the preparation thereof, a culture solution thereof, and a use thereof for the treatment of salivary gland damage
The present invention provides a method for preparing dissolved salivary gland tissue, capable of generating stem cells from salivary glands. The method comprises the steps of: adding collagenase to human salivary gland tissue, followed by shake culture, to dissolve the tissue； homogenizing the dissolved tissue； and adding collagenase and hyaluronidase-1 to the homogenized tissue, followed by shake culture, to hydrolyze glycoside bonds in the tissue. Further, the present invention provides a method for preparing adult stem cells derived from human salivary gland tissue, comprising the step of: adding the dissolved salivary gland tissue, which is prepared by the foregoing method, to a medium containing dexamethasone, mercaptoethanol, oncostatin M, and leukemia inhibitory factor (LIF), followed by culture, and then collecting stem cells from the culture liquid. Further, the present invention relates to adult stem cells derived from human salivary gland tissue, wherein the adult stem cells are prepared by the foregoing method and expresses stem cell type cell surface markers CD44, CD49f, CD90, CD105, Oct-4, and Nestin. Further, the present invention relates to a cell therapeutic agent for treating the damage of salivary glands, comprising, as an active ingredient, the adult stem cells derived from human salivary gland tissue.본 발명은 인간 침샘 조직에 콜라게나제를 가하고 진탕배양 하여 조직을 용해하는 단계, 용해된 조직을 균질화하는 단계, 및 균질화된 조직에 콜라게나제 및 히알루로니다제-1를 가하고 진탕배양 하여 조직 내의 글리코시드 결합을 가수분해하는 단계를 포함하는 침샘줄기세포를 생성시킬 수 있는 용해된 침샘조직을 제조하는 방법； 그 제조방법으로 제조된 용해된 침샘조직을 덱사메타손, 머캅토에탄올, 온코스타틴 M, 및 LIF(leukemia inhibitory factor)을 함유하는 배지에 가하여 배양한 다음, 배양액으로부터 줄기세포를 회수하는 단계을 포함하는 인간 침샘조직 유래 성체 줄기세포를 제조하는 방법； 그 방법으로 제조되고 줄기세포성 세포표면표지인자 CD44, CD49f, CD90, 및 CD105가 발현되는 인간 침샘조직 유래 성체 줄기세포； 및 그 인간 침샘조직 유래 성체 줄기세포를 유효성분으로 포함하는 침샘 손상 치료용 세포치료제를 제공한다.
