An apparatus for non-invasive glucose monitoring includes a light source for emitting at least one ray of light a first beam splitter, a set of photo detectors for measuring optical rotatory distribution (ORD) information and absorption energy information a reference component receiving the light from the first beam splitter, and the light reflected by the reference component being transmitted to the set of photo detectors by the first beam splitter, wherein the light emitted from the light source is transmitted to the set of photo detectors by the first beam splitter and the eyeball to form a first optical path, the light emitted from the light source is transmitted to the set of photo detectors by the first beam splitter and the reference component to form a second optical path and a processing unit receiving and processing the ORD information and the absorption energy information to obtain a glucose information.