A method of preserving a biomaterial according to the present invention includes the step of simultaneously applying minus DC voltage and AC voltage to a biomaterial (O). It is preferable that in the simultaneous voltage application step, the biomaterial (O) is stored in a container (1) that surrounds at least the periphery of the biomaterial (O), and a voltage application plate (2) supplied with minus DC voltage and AC voltage in a superposed manner is placed along one outer surface of the container (1), so that the voltage is indirectly applied to the biomaterial (O).