METHODE DE PRODUCTION DE SACCHAROSE SYNTHASE RECOMBINANTE, SON UTILISATION DANS LA PRODUCTION DE TROUSSE DE DEPISTAGE DE SACCHAROSE, METHODE DE PRODUCTION D'ADP-GLUCOSE ET METHODED'OBTENTION DE PLANTES TRANSGENIQUES AYANT DES FEUILLES ET DES ORGANES DE RESERVE QUI ACCUMULENT UNE CONCENTRATION ELEVEE D'ADP-GLUCOSE ET D'AMIDON
UNIVERSIDAD PUBLICA DE NAVARRA;CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
发明人:
BAROJA FERNANDEZ, MIREN EDURNE,MUNOZ PEREZ, FRANCISCO JOSE,POZUETA ROMERO, FRANCISCO JAVIER,MORAN ZORZANO, MARIA TERESA,ALONSO CASAJUS, NORA
申请号：
CA3002836
公开号：
CA3002836C
申请日:
2005.01.27
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
It is provided a method for the efficient production of large quantities of soluble recombinant sucrose synthase (SS) in the active form thereof, with the expression of the gene that codes for SS in a strain Of Escherichia coll. The expression vector used enables the recombinant SS thus produced to have a histidine tail which facilitates the rapid purification thereof. In addition, it is provided sequences of mutated versions of the SS gene, which code for SS isoforms that are suitable for the production of ADPG. It is also provided a method of obtaining transgenic plants which overexpress the SS gene, either constituently or in the reserve organs or leaves thereof, and which have a high concentration (both in the leaves and in the reserve tissues) of sucrose, ADPG, G6P and starch owing to the high ADPG-synthesising activity of SS.
