An intracellular cytokine flow cytometry (CFC) assay was developed to measure CD3− (non-T) cell response to allo-Ags expressed on peripheral blood mononuclear cells (allo-CFC-PBMC) and/or endothelial cells (allo-CFC-EC) by detecting intracellular gamma-interferon (IFNY) production. The assay can be used to determine a likelihood of antibody mediated rejection in an individual. A method for using genetic screening to determine the likelihood of AMR is also disclosed.
