The Research Foundation for Microbial Diseases of Osaka University
发明人:
FUJIMOTO, Takao,FUJIRA, Junji
申请号：
EP17827625
公开号：
EP3486316A4
申请日:
2017.07.11
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
Provided is a method of producing reassortant influenza virus having genome segments of two or more kinds of influenza virus strains. Specifically, provided is a method of producing reassortant influenza virus containing an antigenic protein of the first influenza virus strain, the method including the following steps: 1) a step of irradiating the first influenza virus strain with ultraviolet light in such an irradiation dose that the first influenza virus strain has initial infection ability and loses or is reduced in virus growth potential； 2) a step of infecting a host with the first influenza virus strain and the second influenza virus strain； 3) a step of culturing the host infected with the first influenza virus strain and the second influenza virus strain, to obtain culture product； 4) a step of inactivating influenza virus strain having an antigenic protein of the second influenza virus strain in the culture product obtained in the step 3)； and 5) a step of collecting reassortant influenza virus after the step 4).
