TOTEY SATISH MAHADEORAO,MANIYAR RACHANA RAJIV,LYLE CARL FONSECA,SNEHA DEEPAK LAGHATE,NICOLE KOSHY
申请号：
IN2406/MUM/2012
公开号：
IN2012MU02406A
申请日:
2012.08.17
申请国别(地区):
IN
年份:
2014
代理人:
摘要：
The invention relates to a method of preparing an intra-dermally, intra-articularly, sub-dermally or topically administrable growth factor concentrate derived from human platelets. The method comprises the steps of suspending human platelets in multiple electrolyte isotonic solution snap-freezing the suspension thawing the frozen suspension and sterile-filtering the suspension. In particular, in this method, a fixed number of platelets is suspended in a fixed volume of multiple electrolyte isotonic solution to obtain the required concentration of growth factors in the growth factor concentrate, snap-freezing of the suspension is carried out at a temperature of -120°C to -200°C, thawing of the frozen suspension is carried out at 25°C to 37°C, and cellular debris are separated from the thawed suspension and the resultant suspension of growth factors is diluted with an isotonic medium before sterile-filtering.
