METHOD OF USING MIXTURES WHICH SIMULATE WHOLE BLOOD AND DIAGNOSTIC SERUMS FOR TEACHING STUDENTS METHODS OF DETERMINING BLOOD GROUPS ACCORDING TO THE SYSTEM AB0 AND RHESUS FACTOR

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专利权人:

Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Petrozavodskij gosudarstvennyj universitet"

发明人:

Volkova Tatyana Olegovna (RU),Волкова Татьяна Олеговна (RU),Ilyukha Vladimir Viktorovich (RU),Илюха Владимир Викторович (RU),Shubin Igor Vladimirovich (RU),Шубин Игорь Владимирович (RU)

申请号：

RU2018146627

公开号：

RU0002712074C1

申请日:

2018.12.25

申请国别(地区):

RU

年份:

2020

代理人:

摘要：

FIELD: medicine.SUBSTANCE: invention relates to biomedicine, specifically to use of mixtures which imitate whole blood and diagnostic α-, β- and α,β-isohaemagglutinating serums for teaching students methods of determining blood groups by system AB0 and Rhesus factor. Use of mixtures which imitate whole blood and diagnostic α-, β- and α,β-isogemagglutinating serums for teaching students methods of determining blood groups by system AB0 and Rhesus factor, including preparation of human and simulator blood simulators α-, β- and α,β-isogamagglutinating serums, where as mixtures which imitate whole blood, use whole or reconstituted milk powder diluted with water in ratio 1:1, water and 2 % solution of sulphosalicylic acid are used as imitators of isohemagglutination serums, then blood group is determined by system AB0 by agglutination reaction, wherein the dye added to the blood simulator is 2 % ponceau solution, and using simulated serum anti-rhesus and the control serum, additionally determining the Rh factor, as anti-rhesus serum 2 % solution of sulphosalicylic acid is used, in its use positive rhesus factor Rh+ is determined, and control serum is water, use of negative rhesus factor Rh- is used to determine rhesus-positive or rhesus-negative blood accessory.EFFECT: method provides 100 % accuracy of determining blood groups and rhesus factor, enables self-implementation of procedures due to their safety, simplicity, reproducibility, uniqueness of interpretation, low cost on laboratory sessions of medical and biological students of universities and colleges.1 cl, 5 tbl, 5 exИзобретение относится к биомедицине, а именно к применению смесей, имитирующих цельную кровь и диагностические α-, β- и α,β-изогемагглютинирующие сыворотки для обучения студентов методикам определения групп крови по системе AB0 и резус-фактора. Применение смесей, имитирующих цельную кровь и диагностические α-, β- и α,β-изогемагглютинирующие сыворотки для обучения студентов методикам определения груп

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