The present invention is intended for the use of a probiotic composition comprising Bifidobacterium animalis subs. lactis (B. lactis), Bifidobacterium longum and Lactobacillus casei, particularly the strains B. lactis CECT 8145, B. longum CECT 7347 and/or L. casei CECT 9104, in the treatment and/or prevention of atopic dermatitis.