A method for spinal drilling operation is disclosed. The method includes the steps of disposing a guiding element on a vertebra, mounting an auxiliary element on a locating part of the guiding element, mounting a k-pin on the auxiliary element, locating the k-pin and removing the auxiliary element from the locating part, mounting a driller having a holding part and a drilling part through the k-pin and rotating the holding part to drive the drilling part for drilling process. The present invention also provides a guiding assembly for spinal drilling operation. The present invention is advantageous for conducting the spinal drilling process easier and more precisely.一種脊椎鑽孔方法,包括以下步驟:設置一導引件於一椎體;套設一輔助件於定位部;套設一導引針於輔助件;固定導引針並自定位部分離輔助件;套設一鑽孔件於導引針,且鑽孔件具有一握持部以及一鑽部;以及旋轉握持部以透過鑽部鑽孔。本發明另提供一脊椎鑽孔之導引組件,利用本發明可簡易且精確地輔助脊椎鑽孔。S41~S46...步驟