A device for mixing biological samples contained in flexible storage bags (104) at controlled temperature, in which the flexible storage bags (104) can serve, in addition to storage, for the collection, frozen storage or transfer of biological samples, comprising the device: a) a support (105) for supporting a storage bag (104) containing a biological sample to be mixed; b) means for imparting a displacement to the sample in the storage bag (104) on the support (105) for mixing the sample; and c) temperature control means for maintaining the sample at a controlled temperature during mixing, in which d) said means for imparting displacement to the sample comprise at least one bag capable of inflating / deflating (102, 103) which, when inflated, it makes contact with the surface of a part of the storage bag (104) to progressively press the storage bag (104) and move the contained samples to another part of the storage bag (104) ; characterized in that: - the support (105) is a base plate (105) on which the storage bag (104) can be placed, - the bag capable of inflating / deflating (102, 103) is placed below a cover (101) that fits on the support (105), the bag capable of inflating / deflating (102, 103) being placed on the location of the supported storage bag (104) and below the cover (101), the cover (101) is pivotally mounted on the support (105) between a closed position and an open position, and - the device further comprises means for locking the cover (101) in the closed position on the support (105) for prevent the cover from opening as a result of inflating the bag capable of inflating / deflating (102, 103) during mixing.Un dispositivo para mezclar muestras biológicas contenidas en bolsas almacenamiento flexibles (104) a temperatura controlada, en el cual las bolsas de almacenamiento flexibles (104) pueden servir, además de almacenamiento, para la recolección, almacenamiento congelado o transferencia de muestras biológicas, comprendiendo el dispositivo: a) u