The present application relates to a method for the prognosis of adverse outcomes in a subject comprising the following steps: (i) determining the midkine level in a sample of said subject； and (ii) comparing the determined midkine level to a control midkine level； wherein said sample is taken after the subject has been subjected to cardiovascular stress； wherein said control midkine level is derived from control sample(s) from one or more subjects not showing adverse outcomes, wherein the control sample(s) have been taken after said one or more subjects not showing adverse outcomes have been subjected to cardiovascular stress； and wherein a decreased determine midkine level as compared to the control midkine level is indicative of an adverse outcome. Further it relates to a method for the prognosis of adverse outcomes in a subject comprising the following steps: (i) determining in samples of said subject the increase in midkine levels (Δ；midkine value) during cardiovascular stress； and (ii) comparing the determined Δ；midkine value to a control Δ；midkine value； wherein said control Δ；midkine value is derived from one or more subjects not showing adverse outcomes； wherein a decreased determined Δ；midkine value as compared to the control Δ；midkine value is indicative of an adverse outcome. It also relates to the use of a midkine antibody or an antigen binding fragment thereof for the prognosis of adverse outcomes in a subject undergoing dialysis therapy. Also encompassed is midkine for use in the treatment or the prevention of an adverse outcome, wherein midkine is administered to the subject.
