The present disclosure relates to a polypeptide having multiple directionality and a self-assembled nanostructure containing the same. Since R1 and R3 domains having β-sheet structures are arranged to have antiparallel structures, it provides a more stabilized α-helix structure than the existing polypeptide having single directionality. In addition, since the polypeptide of the present disclosure is prepared as an antiparallel pseudo-cyclic structure without additional structural element such as a linker, the associated synthesis process is simple and the molecular weight is relatively small.Since the polypeptide having multiple directionality having the structural and functional characteristics described above and a self-assembled nanostructure containing the same have excellent stability and transportability, they are applicable in various fields as drugs, for detection of substances in vivo, for targeting for drug delivery, and for inhibiting protein-mediated biomacromolecular interactions.
