By using it to generate ozone water containing ozone having a particle size which is difficult to disappear in water, to provide a cleaning and sterilizing device which can perform the sterilization of the object to be cleaned such as a medical instrument quickly and effectively. It includes a water purification supplying means, ozone water generating means, and ejection means for ejecting toward the object to be cleaned ozone water and washing water in the container, ozone water generating means, was mixed by incorporating water and ozone a mixing pump for generating ozone mixing water, supplying ozone supply means for ozone mixing pump, the water flow path in a closed, which is located a plurality of protrusions ozone mixed water from the mixing pump, flowing water pressure is applied a stirring means for generating ozone water by miniaturized ozone of ozone mixed water by colliding sequentially a plurality of projections in the, and ozone mixed by circulates stirred stirring means ozone mixed water produced by the mixing pump I provided, and a line mixer to adjust the particle size of the ozone water.水中で消滅し難い粒径のオゾンを含むオゾン水を生成して用いることにより、短時間で効果的に医療器具などの被洗浄物の殺菌を行うことが可能な洗浄殺菌装置を提供する。浄水供給手段と、オゾン水生成手段と、容器内で洗浄水及びオゾン水を被洗浄物に向けて噴射する噴射手段とを備え、オゾン水生成手段が、オゾンと水とを取り込んで混合させたオゾン混合水を生成する混合ポンプと、混合ポンプにオゾンを供給するオゾン供給手段と、混合ポンプからのオゾン混合水を複数の突起が配置された閉じられた流水経路内において、流水圧力が掛けられた状態で複数の突起に順次衝突させることによりオゾン混合水のオゾンを微細化してオゾン水を生成する撹拌手段と、混合ポンプによって生成されたオゾン混合水を撹拌手段に巡回撹拌させることによりオゾン混合水のオゾン粒径を調整するラインミキサと、を備える。