The present disclosure provides a method of producing enhanced amounts of rebaudioside M (Reb M) from various steviol glycosides utilizing the hydrolytic activity of beta-glucosidase. More specifically, the present disclosure provides an improved process to produce desired steviol glycosides from disrupted recombinant cells (e.g., recombinant microbial cells) containing substrates of interest. This is where such cells (e.g., microbes) have been modified to carry genes capable of generating steviol glycosides of interest including rebaudioside A (Reb A), rebaudioside E (Reb E), and/or stevioside. In this embodiment, the process comprises the steps of obtaining the material from disrupted cells (e.g., microbial cells) and carrying out enzymatic hydrolysis on the steviosides present in said waste product to enhance the production of Reb M. Further provided are steviol glycoside compositions produced by the methods described herein.
