Provided herein are anti-infective peptides and uses thereof, in one embodiment, provided herein is a peptide that is 16 to 26 amino acid residues in length and comprises the following amino acid sequence: X1X2SWLRDX3X4TX5LQSX6L, wherein X1 is S or G or A； X2 is S or G, X3 is V or I, X4 is W or L, X5 is W, K, A or L and X6 is W, K, L or A. The anti-infective peptides may comprise D amino acids, L amino acids or a combination thereof. In certain embodiments, the anti-infective peptides are peglyated, modified to include a hydrophilic polymer, stapled or iipidated. Also provided herein are compositions comprising the anti-infective peptides, and methods of treatment and disinfecting using the anti-infective peptides. The anti-infective peptides provided herein are useful against a broad spectrum of bacteria and viruses.
