The invention describes a method for producing growth factors and/or cytokines, a composition and pharmaceutical composition comprising thereof and their uses. The compositions obtained by this method are suitable in medicine regenerative treatments, since are able to regenerate injured tissue. The compositions of the invention may be stored for long periods until its use, avoiding any subsequent blood extraction from the cell-donor, being the stored growth factors and/or cytokines biologically active after storage. Moreover, the compositions may be applied in both autologous and allogenic treatments.
