The present invention provides a human corneal endothelial cell sheet having a curvature fitting a human corneal endothelial surface, particularly, the human corneal endothelial cell sheet supported by a cell support having a curvature fitting a human corneal endothelial surface, and a production method of a human corneal endothelial cell sheet having a curvature fitting a human corneal endothelial surface, which includes the following steps:(1) a step of gelling a gelatin aqueous solution poured into a template having a curvature fitting a human corneal endothelial surface(2) a step of forming a sheet-like gelatin by drying the gelled gelatin obtained in (1)(3) a step of obtaining a gelatin sheet by thermally crosslinking the sheet-like gelatin obtained in (2)(4) a step of seeding human corneal endothelial cells on the gelatin sheet obtained in (3) to form a human corneal endothelial cell layer.
