The invention provides a composite type biological cannula and a preparation method and application thereof. The composite type biological cannula includes a hollow biological catheter and a sustained release micro-capsule layer which is arranged on the inner wall of the hollow biological catheter the inner diameter of the biological catheter is 0.8 mm-9 mm, and the wall thickness of the biological catheter is 0.1-2.5 mm and the thickness of the sustained release micro-capsule layer is 0.05-0.5 mm. The composite type biological cannula is suitable for peripheral nerve interstitium tubulization suture, the sustained release micro-capsule layer on the inner wall of the cannula can sustainably provide nerve growth substances, and the restoration effect of damaged peripheral nerves can be improved.
