PROBLEM TO BE SOLVED: To provide a means capable of efficiently growing a corneal endothelial cell and a means to stably supply a corneal endothelial preparation.SOLUTION: There are provided: an agent for promoting adhesion of a corneal endothelial cell, comprising a Rho kinase inhibitor； a culture medium for a corneal endothelial cell, comprising the Rho kinase inhibitor； a corneal preservation solution comprising the Rho kinase inhibitor； a method of producing a corneal endothelial preparation, comprising a step of culturing the corneal endothelial cell using the culture medium； and an agent for protecting corneal endothelium, comprising the Rho kinase inhibitor.【課題】角膜内皮細胞を効率的に増殖可能な手段および角膜内皮製剤の安定的供給手段の提供。【解決手段】Ｒｈｏキナーゼ阻害剤を含有してなる角膜内皮細胞の接着促進剤、Ｒｈｏキナーゼ阻害剤を含有してなる角膜内皮細胞の培養液、Ｒｈｏキナーゼ阻害剤を含有してなる角膜保存液、前記培養液を用いて角膜内皮細胞を培養する工程を含む、角膜内皮製剤の製造方法、Ｒｈｏキナーゼ阻害剤を含有してなる角膜内皮保護剤。【選択図】なし
