The present invention relates to a combined norovirus and rotavirus vaccine for prevention of norovirus and rotavirus infection and/or viral-induced diarrheal and vomiting diseases in man. More specifically, the invention comprises a method of preparing combination vaccine compositions comprising norovirus and rotavirus antigens, in particular mixtures of norovirus VLPs and rotavirus recombinant VP6 protein or double-layered VP2/VP6 VLPs. In addition, the invention relates to methods of inducing an immune response.本發明關於用於抑制人類諾羅病毒與輪狀病毒感染及/或病毒引起之腹瀉與嘔吐疾病的諾羅病毒與輪狀病毒組合疫苗。更特別是,本發明包括一種製備包含諾羅病毒與輪狀病毒抗原之組合疫苗組合物的方法,特別是,諾羅病毒VLPs與輪狀病毒VP6或雙層VP2/VP6 VLPs的混合。本發明也有關於一種誘導免疫反應的方法。