The invention relates to a duck viral enteritis virus infective cloned system, which is characterized in that the system comprises multiple cosmids, each cosmid possesses clone of a gene fragment of duck viral enteritis viral vaccine strain, the gene fragment of the duck viral enteritis viral vaccine strain contains a mutual overlapped area, and splices to cover the whole gene group of the duck viral enteritis viral vaccine strain. The invention also relates to a construction method and an application of the duck viral enteritis virus infective cloned system.