TRUBION PHARMACEUTICALS, INC.;LEDBETTER, JEFFREY, A.;HELLSTROM, INGEGERD;HAYDEN-LEDBETTER, MARTHA;HELLSTROM, KARL, ERIK
发明人:
LEDBETTER, JEFFREY, A.,HELLSTROM, INGEGERD,HAYDEN-LEDBETTER, MARTHA,HELLSTROM, KARL, ERIK
申请号：
WO2003US09415
公开号：
WO03083069(A8)
申请日:
2003.03.26
申请国别(地区):
世界知识产权组织国际局
年份:
2005
代理人:
摘要：
An improved method for ex-vivo generation of tumor-reactive T cells is provided, comprising, for example, culturing PBMC from a patient with cancer with autologous tumor cells and magnetic beads that can activate T lymphocytes via CD3 in combination with CD28, CD40, or CD28 plus CD40. The invention also provides genes encoding anti-CD3 or anti-4??1BB scFv and methods to transfect cells of neoplastic or normal origin for expression of those genes at their surface. The genes and transfected tumor cells are useful compositions for induction of a tumor-destructive immune response.
