PURPOSE: A cosmetic composition including N-acetyl-L-glutamine is provided to prevent skin aging and improve wrinkles by eliminating oxidizing substances, biosynthesizing collagen, promoting cell proliferation and keratin hydration. CONSTITUTION: A cosmetic composition including N-acetyl-L-glutamine contains N-acetyl -L- glutamine as active ingredients. 0.1-15 weight% of the N-acetyl-L-glutamine is included based on the total cosmetic composition. The cosmetic composition additionally includes an emulsifier. The emulsifier is monooleic acid ester or glycerol monostearin acid ester. The cosmetic composition having oxidizing substances elimination effects includes the N-acetyl -L- glutamine as active ingredient. The cosmetic composition having collagen biosynthesis effects includes the N-acetyl -L- glutamine as active ingredient. A cosmetic composition having cell proliferation promotion effects includes the N-acetyl -L- glutamine as active ingredient. The cosmetic composition having the keratin hydration acceleration effect includes the N-acetyl -L- glutamine as active ingredient.본 발명은 N-아세틸-L-글루타민을 유효성분으로 포함하는 화장료 조성물에 관한 것으로, 구체적으로는 N-아세틸-L-글루타민을 유효성분으로 포함함으로써 산화물질 소거, 콜라겐 생합성, 세포 증식 촉진, 각질 수화 촉진을 통하여 피부의 노화를 억제하고 주름을 개선하는 효과가 있는 화장료 조성물에 관한 것이다.