This invention relates to compositions and methods for cleaning, disinfection, and sterilization of hard, soft, and porous surfaces, equipment, human skin, tissues, food and vegetable surfaces, and other media which are contaminated with microorganisms such as bacteria, viruses, yeast, and molds. Particularly relevant is the microbial environment whereby bacterial communities can generate three-dimensional polymicrobial Extracellular Polymeric Substances, or EPS, biofilm communities supported by an aggregation of microorganisms growing on a substrate layer, substantially degrading the antimicrobial performance of typical cleaners, disinfectants, and sterilizers. There is great need for a composition that will reliably eradicate microorganisms across a broad spectrum, particularly polymicrobial EPS communities shrouded by self-generated biofilm layer. Such a composition is disclosed herein. The invention discloses new compositions, and methods for formulation of compositions, of weak acids, surfactants, and glycol monoesters, which achieve a level of eradication such that when tested following application, no surviving microorganisms remain. All compositions and methods disclosed herein have the further benefit of their ingredients being classified by the US Food and Drug Administration (FDA) as GRAS, or Generally Regarded As Safe for use on food and food contact surfaces； and the embodiment of the claimed invention herein contains 95% USDA Certified Biobased content, and is accepted to the USDA BioPreferred® Program.
