Some embodiments of the present inventive concept provide a multispectral imaging system including a first light source, the first light source having a first wavelength configured to produce a non-coherent illumination to image a sample； a second coherent light source, different from the first light source, having a second wavelength, different from the first wavelength, configured to image the sample simultaneously with the first light source； a camera configured to simultaneously receive information related to the first and second light sources from the sample, wherein light at the first wavelength is configured to image a surface of the sample into the camera and light at the second wavelength is configured to penetrate the sample and provide information related to the penetrated sample to the camera； and a processor configured to combine the received information related to the first and second light sources and generate a synthesized image of the anatomical structure and the physiology of blood flow and perfusion of the sample in terms of blood flow rate distribution.
