The present invention relates to a use of zingiber officinale extract in inhibition of colon cancer cell proliferation and growth cycle. Further speaking, hexahydrocurcumin can be extracted from zingiber officinale and used to inhibit the proliferation and growth cycle of colon cancer cells.本發明係有關於一種生薑萃取物在抑制大腸癌細胞生長與細胞週期性之用途,係由生薑中萃取出六氫薑黃素(hexahydrocurcumin),並用於抑制大腸癌細胞生長與細胞週期性之用途。