To provide a pharmaceutical composition comprising a peptide-polynucleotide complex, and methods of use thereof.SOLUTION: The invention provides the use of a pharmaceutical composition comprising a peptide-polynucleotide complex, the peptide-polynucleotide complex comprising a ratio of peptide:polynucleotide that is more than about 50:1 and less than about 200:1, where the peptide is characterized by (a) being non-lytic and capable of affecting the release of a polynucleotide from an endosome of a cell and (b) comprising an amino acid sequence with at least 80% identity to a specific amino acid sequence.SELECTED DRAWING: None【課題】ペプチド－ポリヌクレオチド複合体を含む薬学的組成物、及びその使用方法の提供。【解決手段】ペプチド－ポリヌクレオチド複合体が、約５０：１を超え、約２００：１未満のペプチド：ポリヌクレオチドの比率を有し、前記ペプチドが（ａ）非溶解性であり、細胞のエンドソームからのポリヌクレオチドの放出に影響を及ぼすことができ、かつ（ｂ）特定のアミノ酸配列に対して少なくとも８０％の同一性を有するアミノ酸配列を含む、薬学的組成物の使用。【選択図】なし
