Katalin Kariko,Drew Weissman,Gary Dahl,Anthony Person,Judith Meis,Jerome Jendrisak
申请号：
US12962468
公开号：
US09012219B2
申请日:
2010.12.07
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.
