Trehalose azelaic acid ester is disclosed. A preparation method of the trehalose azelaic acid ester includes following steps: providing a mixing step, providing an esterification step, providing a separation step and providing a purification step to obtain the trehalose azelaic acid ester. Furthermore, uses of the trehalose azelaic acid ester are disclosed. The trehalose azelaic acid ester is used for inhibiting the growth of bacteria and preparing skin whitening drugs, antioxidative drug, antihypertensive drug and neuroprotection drug.本發明提供一種海藻糖壬二酸酯,其製造方法至少包含下述步驟:進行一混合步驟、進行一酯化反應、進行一分離步驟及進行一純化步驟以獲得海藻糖壬二酸酯。本發明亦提供海藻糖壬二酸酯之應用,係用於抑制細菌生長、製備美白之藥物、製備抗氧化之藥物、製備降血壓之藥物及保護神經之藥物。100‧‧‧海藻糖壬二酸酯製造方法110‧‧‧步驟120‧‧‧步驟130‧‧‧步驟140‧‧‧步驟