Janina Ratajczak,Ewa K. Zuba-Surma,Mariusz Ratajczak
申请号：
US13129359
公开号：
US20120114614A1
申请日:
2009.11.16
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Methods for isolating a CD133+/CD45neg/GlyAneg subpopulation of umbilical cord blood cells are disclosed. In some embodiments, the methods include providing an initial population of umbilical cord blood cells contacting the initial population of cells with a first antibody that is specific for CD133, a second antibody that is specific for CD45, and a third antibody that is specific for Glycophorin A (GIyA) under conditions sufficient to allow binding of each antibody to its target, if present, on each cell of the initial population of cells and isolating a subpopulation of cells that are CD133+, CD45neg, and GlyAneg. Also provided are isolated populations of CD133+/GlyAneg/CD45neg stem cells isolated from cord blood, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for inducing hematopoietic competency in CD133+/GlyAneg/CD45neg stem cells, and cell culture systems that include CD133+/GIyAneg/CD45neg stem cells.
