Yoon-Sun Yang,Won II Oh,Sun Jae Kwon,Mi Yeon Lee,Hong Bae Jeon
申请号：
US14425220
公开号：
US20150284686A1
申请日:
2013.09.02
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5% oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity, but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.
