Disclosed is a composition of matter comprising an isolated polypeptide, which is a peripherally-restricted NaV1.7 inhibitor. In some disclosed embodiments, the isolated polypeptide is an inhibitor of NaV1.7 and/or NaV1.3. Other embodimentsare conjugated embodiments of the inventive composition of matter and pharmaceutical compositions containing the inventive composition of matter. Isolated nucleic acids encoding some embodiments of inventive polypeptides and expression vectors, and recombinant host cells containing them are disclosed. A method of treating or preventing pain is also disclosed.本發明揭示一種包含經分離多肽之目標組合物，該組合物為周邊限制性NaV1.7抑制劑。在一些所揭示之實施例中，經分離多肽為NaV1.7及/或NaV1.3之抑制劑。其他實施例為本發明目標組合物的結合實施例及含有本發明目標組合物之醫藥組合物。揭示編碼本發明多肽之一些實施例的經分離核酸及表現載體，及含有其之重組宿主細胞。亦揭示一種治療或預防疼痛之方法。
