PROBLEM TO BE SOLVED: To provide a method for producing a seedling of brown algae, the method comprising: greatly increasing the probability of growing into a seedling of brown algae to satisfactorily produce seedlings in large quantities, and allowing the production efficiency of seedlings of brown algae to be extremely increased.SOLUTION: The present invention provides a method for producing a seedling of brown algae, the method comprising: a tissue fragment-collecting step for collecting a tissue fragment 2 from brown algae, such as kelps and gulfweed a callus-forming step for forming a callus 3 on this tissue fragment 2 by culturing the tissue fragment 2 collected in the tissue fragment-collecting step in a culture medium to which a gibberellin synthesis inhibitor is added, i.e., a culture medium supplemented with a gibberellin synthesis inhibitor and a seedling-forming step for forming a seedling 5 by culturing the tissue 2 on which the callus 3 is formed in the callus-forming step in a culture medium to which a gibberellin synthesis inhibitor is not added, i.e., a culture medium free of a gibberellin synthesis inhibitor.SELECTED DRAWING: Figure 2COPYRIGHT: (C)2017,JPO&INPIT【課題】褐藻類の種苗へと生長する確率を非常に高くして、種苗を良好にかつ大量に生産できることにより、褐藻類の種苗の生産効率を極めて良いものにすることができる褐藻類の種苗生産方法を提供する。【解決手段】コンブ類やホンダワラ類等の褐藻類から組織片2を採取する組織片採取工程と、この組織片採取工程において採取した組織片2をジベレリン合成阻害剤を添加したジベレリン合成阻害剤添加培地にて培養し、この組織片2にカルス3を形成するカルス形成工程と、このカルス形成工程においてカルス3を形成した組織片2をジベレリン合成阻害剤を添加していないジベレリン合成阻害剤無添加培地にて培養して種苗5を作製する種苗作製工程と、を備えた褐藻類の種苗生産方法である。【選択図】図2