A controlled environment enclosure comprises a robotic armmanipulation system used to protect and unprotect a fluid path and a swabwithin the controlled environment enclosure. The apparatus allows the fluidpath to be protected against dangerous decontamination vapors and chemicalsbefore the controlled environment enclosure is decontaminated. The apparatusallows the fluid path to be unprotected without the use of gloves orother means that degrade the integrity of the controlled environment enclosurewhen decontamination is completed. The apparatus and method allowfor the protecting, unprotecting and decontaminating sequences to beautomated.In some embodiments the fluid path comprises a fill needle that canremovably and aseptically be sealed with a disposable monolithic injectionmoulded polymeric fill needle sheath. The apparatus and method further allowfor the use of a swab disposed in a swab holder that is aseptically andremovably sealable to a swab cap to protect the swab against decontaminationvapors.
