PROBLEM TO BE SOLVED: To provide a technique for cutting out a cell membrane from an animal cell by a simple method.SOLUTION: The invention provides a technique comprising a pretreatment step of immersing an animal cell into a first aqueous solution containing a monovalent and/or divalent metal ion of high concentration as compared with concentration in vivo, a cutting out step of immersing the pretreated animal cell into the aqueous solution corresponding to a hypotonic solution to the animal cell after the immersion.COPYRIGHT: (C)2015,JPO&INPIT【課題】動物細胞から簡便な方法で細胞膜を切り出す技術を提供する。【解決手段】動物細胞を、生体内と比較して高い濃度の1価及び/又は2価の金属イオンを含む第1の水溶液に浸漬する前処理行程と、前記浸漬後の細胞に対して低張液に該当する水溶液に前処理された動物細胞を浸漬する切り出し行程を含む。【選択図】図1