WISCONSIN ALUMNI RESEARCH FOUNDATION;BREMEL, ROBERT, D.;BLECK, GREGORY, T.
发明人:
BREMEL, ROBERT, D.,BLECK, GREGORY, T.
申请号：
WO1999US26848
公开号：
WO0030437(A8)
申请日:
1999.11.17
申请国别(地区):
世界知识产权组织国际局
年份:
2001
代理人:
摘要：
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
