INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE INSERM
发明人:
ABERDAM DANIEL,CORAUX CHRISTELLE
申请号：
FR20010007167
公开号：
FR2825370(A1)
申请日:
2001.05.31
申请国别(地区):
法国
年份:
2002
代理人:
摘要：
The invention concerns a method for inducing keratinocyte differentiation of embryonic stem cells of mammals, comprising the following steps: isolating an extracellular matrix secreted by at least one type of mammalian cells; culturing in parallel embryonic stem cells of mammals in undifferentiated state in a suitable culture medium in the presence of LIF; seeding the embryonic stem cells in monolayer on said extracellular matrix; culturing the thus seeded embryonic stem cells in the absence of LIF for a time interval sufficient for their keratinocyte differentiation; and collecting the thus obtained keratinocytes.
