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Engineered landing pads for gene targeting in plants
专利权人:
DOW AGROSCIENCES LLC
发明人:
MILES REBECCA RUTH,AINLEY WILLIAM MICHAEL,CORBIN DAVID RICHARD,BLUE RYAN C,MURRAY MICHAEL G,WEBB STEVEN R
申请号:
NZ60073311
公开号:
NZ600733A
申请日:
2011.01.21
申请国别(地区):
NZ
年份:
2014
代理人:
摘要:
Disclosed is a method for producing a transgenic plant or plant tissue, the method comprising: providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lacks sequence homology with genomic DNA of a host plant cell, and at least one targeting endonuclease recognition site, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the host plant cell flank the at least one targeting endonuclease recognition site, and wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the host plant cell do not comprise an open reading frame and may comprise one of more of the nucleotide sequences of SEQ ID NOs: 1-10 wherein in the sequences are as disclosed in the specification transforming the host plant cell or plant tissue comprising the host plant cell with the nucleic acid molecule, wherein the nucleic acid molecule is stably integrated into the genome of the host plant cell and generating plant tissue or regenerating a plant from the plant cell transformed with the nucleic acid molecule.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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