RECEPTEURS GUSTATIFS HETERO-OLIGOMERES T1R, LIGNEES CELLULAIRES EXPRIMANT LESDITS RECEPTEURS ET UTILISATION DE CES RECEPTEURS POUR IDENTIFIER DES COMPOSES GUSTATIFS
ZOLLER, MARK T.,LI, XIAODONG,STASZEWSKI, LENA,O'CONNELL, SHAWN,ZOZULYA, SERGEY,ADLER, JON ELLIOTT,XU, HONG,ECHEVERRI, FERNANDO
申请号：
CA2451317
公开号：
CA2451317C
申请日:
2002.06.26
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention relates to the discovery that the T1R receptors assembleto form functional taste receptors. Particularly, it has been discovered thatco-expression of T1R1 and T1R3 results in a taste receptor that responds toumami taste stimuli, including monosodium glutamate. Also, it has beendiscovered that co-expression of the T1R2 and T1R3 receptors results in ataste receptor that responds to sweet taste stimuli including naturallyoccurring and artificial sweeteners. Also the present invention relates to theuse of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 inassays to identify compounds that respectively respond to umami taste stimuliand sweet taste stimuli. Further, the invention relates to the constitutive ofcell lines that stably or transiently co-express a combination of T1R1 andT1R3； or T1R2 and T1R3； under constitutive or inducible conditions. The use ofthese cells lines in cell-based assays to identify umami and sweet tastemodulatory compounds is also provided, particularly high throughput screeningassays that detect receptor activity by use of fluorometric imaging. Finally,the invention relates to the discovery that some compounds, e.g., lactisole,inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, andaccordingly the sweet and umami taste, suggesting that these receptors may bethe only sweet and umami receptors.
