The present invention relates to Grifola frondosa extract having skin whitening effect, its manufacturing method and its applications thereof. The manufacturing method of the Grifola frondosa extract comprises the steps of preparing Grifola frondosa drying the Grifola frondosa to a Grifola frondosa powder adding a solvent into the Grifola frondosa powder to form a Grifola frondosa solution sonicating the Grifola frondosa solution and waiting for a desired time to let the Grifola frondosa solution layering to obtain an upper solution centrifugating the upper solution to obtain supernatant and filtering the supernatant to gain a Grifola frondosa extract. The Grifola frondosa extract has functions of removal of DPPH free radicals, anti-oxidation and anti-tyrosine activity. Therefore, the Grifola frondosa extract has the ability to whitening skin and can be used as an additive in related products.本發明係有關於一種具有美白功能之舞菇萃取液、其製備方法及其應用,該舞菇萃取液之製備方法,其包含:提供一舞菇原料;將該舞菇原料烘乾製成一舞菇粉末;將該舞菇粉末加入一溶劑中,形成一舞菇溶液;將該舞菇溶液以超音波震盪,並靜置一預定時間,待該舞菇溶液分層後,取其上層液;離心該上層液,以取得一上清液;以及過濾該上清液,即得一舞菇萃取液。該舞菇萃取液經測試證實具有清除DPPH自由基能力、抗氧化能力以及抗酪氨酸酵素活性能力,故具有良好之美白功效並可添加於相關產品中。S11至S16‧‧‧步驟