Quinn P Peterson,Felicia J Pagliuca,Douglas A Melton,Jeffrey R Millman,Michael Saris Segel,Mads Gurtler
申请号：
GB201622354
公开号：
GB2549822A
申请日:
2014.06.11
申请国别(地区):
GB
年份:
2017
代理人:
摘要：
Differentiated non native pancreatic b cells and methods of differentiating progenitor cells to such cells A composition comprising a non-native pancreatic β cell, wherein :(a) the non-native pancreatic β cell comprises one or more crystalline insulin granules； (b) the non-native pancreatic β cell expresses the following genes: INS, PDX1, NKX6-1, and ZNT8； and (c) the non-native pancreatic β cell exhibits an in vitro glucose-stimulated insulin secretion response to a first glucose challenge and a second glucose challenge when the first glucose challenge and the second glucose challenge are applied sequentially is claimed. Methods of preparing such cells using media comprising a thyroid hormone signalling pathway activator such as triiodothyronine and a transforming growth factor b (TGF-b) signalling pathway inhibitor, such as Alk5 inhibitor II are claimed.
