ALKAN, Ozan,KERR, Douglas Anthony,KOTIN, Robert Michael,KLATTE, Debra,LIU, Leah,SILVER, Nathaniel
申请号：
SG11202006431W
公开号：
SG11202006431WA
申请日:
2019.02.14
申请国别(地区):
SG
年份:
2020
代理人:
摘要：
The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of an antibody or fusion protein in a cell, tissue or subject. Such antibodies or fusion proteins can be expressed for treating disease or alternatively, for the production of antibodies or fusion proteins in a commercial setting.
