PREPARATION OF POLYOVIRUS AT HIGH TITRES FOR VACCINE PREPARATION

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专利权人:

CRUCELL HOLLAND B.V.

发明人:

LEWIS, JOHN, ALFRED

申请号：

DK10729895

公开号：

DK2454364T3

申请日:

2010.07.08

申请国别(地区):

DK

年份:

2014

代理人:

摘要：

Provided is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.

来源网站：

中国工程科技知识中心

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http://www.ckcest.cn/home/