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PREPARATION OF POLYOVIRUS AT HIGH TITRES FOR VACCINE PREPARATION
专利权人:
CRUCELL HOLLAND B.V.
发明人:
LEWIS, JOHN, ALFRED
申请号:
DK10729895
公开号:
DK2454364T3
申请日:
2010.07.08
申请国别(地区):
DK
年份:
2014
代理人:
摘要:
Provided is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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