The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells； (b) preparation of protoplasts from said heterologous population of plant cells； (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting； (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material； (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.本發明提供一種由植物細胞的異源性群體產生單株植物細胞株的方法，其包含下列步驟：(a)提供植物細胞的異源性群體；(b)由該植物細胞的異源性群體製備原生質體；(c)藉由將原生質體的製備物進行流式細胞分選而分離單獨原生質體；(d)於餵食細胞材料存在的情況下，藉由共培育使分離的單一經轉形原生質體再生直到形成小聚落；(e)將小聚落自餵食細胞材料移去並培育小聚落直到形成單株植物細胞株。
