HORIZON DISCOVERY LIMITED;MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN;GENETIC INFORMATION RESEARCH INSTITUTE
发明人:
BUERCKSTUEMMER, Tilmann,KAPITONOV, Vladimir Vyacheslavovich,GRABUNDZIJA, Ivana,IVICS, Zoltan
申请号：
SG11201806400X
公开号：
SG11201806400XA
申请日:
2017.02.10
申请国别(地区):
SG
年份:
2018
代理人:
摘要：
The invention relates to a system and method for introducing DNA into cells. In particular, the invention relates to a method for introducing single or multiple copies of a DNA sequence or gene of interest into a cell comprising providing: a) a “copy and paste” transposase； and b) a construct comprising a DNA sequence or gene of interest flanked by a “copy and paste” transposon terminal sequence, such as an LTS or RTS. A novel “copy and paste” transposon of the Helitron family is described along with systems for using the corresponding transposase in methods for introducing DNA into cells, for example, to generate cell lines for use in protein production, cell and gene therapy or as reference standards.
