PIONEER HI-BRED INTERNATIONAL, INC. ;E. I. DU PONT DE NEMOURS AND COMPANY
发明人:
Cigan Andrew Mark,Falco Saverio Carl,Gao Huirong,Lassner Michael,Jantz Derek,Li Zhongsen,Liu Zhan-Bin,Svitashev Sergei,Smith James Jefferson
申请号：
US201816007529
公开号：
US2018305706(A1)
申请日:
2018.06.13
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
