A method for filling containers with fresh blood components, comprising the following steps of: a) providing a piece of equipment (1) defining a transit channel (3) having at least a first gap (3a) associated with a bag (5) or the like containing a fresh blood component, at least a second gap (3b) connected to a plurality of containers (6) to be filled, and at least a third gap (3c) connected to pumping means (12); b) isolating the first gap (3a) from the second gap (3b) and placing the latter in communication with the third gap (3c); c) suctioning the air contained in the containers (6) to be filled by means of the pumping means (12) so as to define a vacuum inside them; d) placing the first gap (3a) in communication with the second gap (3b), the containers (6) suctioning the contents of the bag (5) by effect of the vacuum defined inside them; and where the channel (3) has at least a fourth gap (3d) associated with an empty syringe (4) or the like, and by the fact that it comprises, after step d), the following steps of: e) isolating the first gap (3 a) from the second gap (3b) and placing the first gap itself in communication with the fourth gap (3d); f) taking at least a part of the contents of the bag (5) by means of the syringe (4); g) isolating the fourth gap (3d) from the first gap (3a) and placing the fourth gap itself in communication with the second gap (3b); h) sending the contents taken with the syringe (4) into the containers (6).